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In our experiments, the dead cells could be visualized by the live cell fluorescence microscopy, while the apoptotic cells were not stained by PI. The dead or apoptotic cells were detected by PI. In this device, cells or cell aggregates were cultured in the chip, then exposed to drugs. A three-dimensional microfluidic device was constructed and used to study the interaction between the cells and drug at the same time. In the present work, apoptosis induced by the anticancer drug bleomycin was studied by using the combination of three-dimensional (3D) microfluidic device and live cell fluorescence microscopy.
Although it can be induced by chemicals, drugs or radiations, the detailed mechanism of apoptosis still remains unclear. Live cell fluorescence microscopy in combination with microfluidic device for studies of drug-induced apoptosis.Īpoptosis, the programmed cell death, is involved in various human diseases. You can then use VLC to play most videos (MPEG-1, MPEG-2, MPEG-4, DivX, H.264, Matroska, Ogg, Theora, WebM, RealAudio/Video,…) and movies (Matroska, Ogg Theora, WebM, RealMedia, QuickTime,…) on your computer.
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